Fig. 8
From: CircHYBID regulates hyaluronan metabolism in chondrocytes via hsa-miR-29b-3p/TGF-β1 axis
CircHYBID upregulates the expression of hsa-miR-29b-3p target gene—transforming growth factor (TGF)-β1. A Dual-luciferase reporter assays confirmed the potential binding site of TGF-β1 and hsa-miR-29b-3p. B The relative mRNA expression of TGF-β1 in IL-1β-induced chondrocytes with or without overexpressed circHYBID was analyzed using quantitative real-time polymerase chain reaction (PCR). C The protein expression of TGF-β1 in IL-1β-induced chondrocytes with or without overexpressed circHYBID was analyzed using Western blotting. D The relative mRNA expression of TGF-β1 in IL-1β-induced chondrocytes with overexpressed circHYBID or miR-29b-3p mimic treatment was analyzed using quantitative real-time PCR. E The protein expression of TGF-β1 in IL-1β-induced chondrocytes with overexpressed circHYBID or miR-29b-3p mimic treatment was analyzed using Western blotting. F The expression of TGF-β1 in paired samples of intact and damaged cartilage was analyzed using immunohistochemistry. Magnification: × 200, Scale bar = 50 μm. G IHC score of TGF-β1. H Correlation scatter plot of relative circHYBID expression and score of TGF-β1 (Pearson’s correlation, r = 0.5489), and correlation scatter plot of IHC score of TGF-β1 with alcian blue score (Pearson’s correlation, r = 0.6612). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001