Fig. 3

MALAT1 inhibited the expression of miR-144 to increase the expression of GSK3β. A Venn diagram showing the intersection of StarBase (http://starbase.sysu.edu.cn/), RNAInter (http://www.rna-society.org/rnainter/, Score ≥ 0.99), LncBase (http://carolina.imis.athena-innovation.gr/diana_Tools/web/Index.php?R=lncbasev2%2Findex, Score ≥ 0.9) results using the jvenn online tool (http://jvenn.toulouse.inra.fr/app/example.html). B Expression of miR-144 in lung tissues of mice as detected by RT-qPCR (8 mice in each group; *p < 0.05, compared with that of the sham group; ^#p < 0.05, compared with the I/R group). C Expression of miR-144 in cells as detected by RT-qPCR (*p < 0.05, compared with the control group; ^#p < 0.05, compared with the H/R group). D MALAT1 silencing efficiency as determined by RT-qPCR (***p = 0.0001, compared with the sh-NC group; ****p < 0.0001, compared with the sh-NC group). E Expression of miR-144 in cells as determined by RT-qPCR (*p < 0.05, compared with the sh-NC group; ^#p < 0.05, compared with the oe-NC group). F Binding sites between MALAT1 and miR-144 as predicted by StarBase. G Binding relationship between MALAT1 and miR-144 as verified by dual-luciferase reporter gene assay (*p < 0.05, compared with mimic NC). H Binding of MALAT1 to miR-144 as measured by RNA pull-down (*p < 0.05, compared with Bio-NC). I Venn diagram showing the intersection of StarBase, mirDIP (http://ophid.utoronto.ca/mirDIP/, Score > 0.5), TargetScan (http://www.targetscan.org/vert_72/), RNAInter (http://www.rna-society.org/rnainter/, Score > 0.6) and microT (http://diana.imis.athena-innovation.gr/DianaTools/index.php?R=microT_CDS/, Score > 0.9) results and I/R-related genes. J Co-expression network as plotted using GeneMANIA tool (http://genemania.org/). K Expression of GSK3β in mice as assessed by immunohistochemistry (8 mice each group; *p < 0.05, compared with that of the sham group; ^#p < 0.05, compared with the I/R group). L miR-144 overexpression and silencing efficiencies (*p < 0.05, compared with mimic NC; ^#p < 0.05, compared with inhibitor NC). M Expression of GSK3β in cells as assessed by Western blotting (*p < 0.05, compared with mimic NC; ^#p < 0.05, compared with inhibitor NC). N Binding sites and mutant sites between miR-144 and GSK3β. O Binding relationship between miR-144 and GSK3β as verified by dual-luciferase reporter gene assay (*p < 0.05, compared with mimic NC). P GSK3β expression in cells as determined by RT-qPCR (*p < 0.05, compared with mimic NC). Q GSK3β expression in cells as determined by Western blotting (*p < 0.05, compared with mimic NC + oe-NC). Cellular experiments were repeated 3 times independently