Fig. 5

Overexpressed GSK3β inhibits protective effects of Propofol on H/R cells. A Autophagy-related protein expression in cells as detected by Western blot analysis (*p < 0.05, compared with that of the control group; ^#p < 0.05, compared with the H/R group). B Autophagosome amount in cells as measured by immunofluorescence against LC3 (*p < 0.05, compared with that of the control group; ^#p < 0.05, compared with the H/R group). C Inflammatory factor levels in cells as detected by ELISA (*p < 0.05, compared with the control group; ^#p < 0.05, compared with the H/R group). D GSK3β overexpression efficiency as detected by Western blot analysis. E, Expression of GSK3β and autophagy-related protein levels as determined by Western blot analysis (*p < 0.05, compared with that of the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). F Autophagosome amount in cells as measured by immunofluorescence against LC3 (*p < 0.05, compared with the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). G Cell apoptosis as assessed by flow cytometry (*p < 0.05, compared with the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). H, Inflammatory factor levels as determined by ELISA (*p < 0.05, compared with the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). The experiment was repeated 3 times independently