Fig. 2

Differential Dynamic Network Analysis of inflammatory mediators in the heart of C57BL/6 and TLR4^−/− mice. Animals were injected with LPS (3 mg/kg, i.p.). At different time points upon sacrifice the heart was collected and inflammatory mediators were measured by Luminex™. DyNA (stringency level = 0.7) was then performed during each of the following five time-frames: 1–4 h, 4–6 h, 6–12 h, 12–24 h, and 24–48 h as described in Materials and Methods. Figure shows an overview of all the individual networks and mediator connections in the heart of C57BL/6 mice (A, top row) and the detailed connectivity for TNF (blue arrows) during each of the five time-frames analyzed (A, bottom row). The same analysis for the heart of TLR4^−/− mice is shown in (B)