Fig. 3

Dynamic Network Analysis of inflammatory mediators shows different inflammatory connectivity in C57BL/6 vs. TLR4^−/− mice. Animals were injected with LPS (3 mg/kg, i.p.). At different time points upon sacrifice, blood and different organs (liver, heart, gut, lung, spleen and kidney) were collected, inflammatory mediators were measured by Luminex™ and DyNA (stringency level = 0.7) was performed during each of the following five time frames: 1–4 h, 4–6 h, 6–12 h, 12–24 h, and 24–48 h as described in Materials and Methods. A shows the total number of connections for plasma and different organs. The mediator connection ratio (C57BL/6:TLR4^−/−) calculated from the total number of connections is shown in the inset. B shows the sum of AUCs for all mediators calculated over 0–48 h in heart, plasma, lung, spleen, kidney, liver and gut. The inset depicts the ratio of AUCs (C57BL/6:TLR4^−/−) for plasma and different organs calculated from the sum of AUCs for all mediators shown in the main figure