Table 1 Primer sequence
Gene | Primer sequence (5′–3′) | GenBank number |
|---|---|---|
miR-135 | Forward: 5′-GCTTATGGCTTTTCATTCCT-3′ | NR_029893.1 |
Reverse: 5′-GTGCAGGGTCCGAGGT-3′ | ||
U6 | Forward: 5′-CTCGCTTCGGCAGCACATATACTA-3′ | NR_002752.2 |
Reverse: 5′-ACGAATTTGCGTGTCATCCTTGCG-3′ | ||
PCSK6 | Forward: 5′-ACTCCAGAAGAAGAGGAAGAGTA-3′ | NM_138319.4 |
Reverse: 5′-ACCATCGCAGCCTTTATCA-3′ | ||
NLRP3 | Forward: 5′-CCAGGGCTCTGTTCATTG-3′ | NM_001191642.1 |
Reverse: 5′-CCTTGGCTTTCACTTCG-3′ | ||
Bcl‐2 | Forward: 5′‐ATGTGTGTGGAGAGCGTCAA‐3′ | NM_000633.2 |
Reverse: 5′-ACAGTTCCACAAAGGCATCC-3′ | ||
Bax | Forward: 5′‐GGGGACGAACTGGACAGTAA‐3 | NM_004324.3 |
Reverse: 5′-CAGTTGAAGTTGCCGTCAGA-3′ | ||
GAPDH | Forward: 5′-TTGTGTCCGTCGTGGATCTGA-3′ | NM_008084.2 |
Reverse: 5′-TTGCTGTTGAAGTCGCAGGAG-3′ |