Fig. 1

Pref-1 and PEA3 expression in lung tissue sections obtained from OVA-treated mice. A Mice were sensitized with OVA through three intraperitoneal injections on days 1, 7, and 14 with OVA absorbed to aluminium hydroxide. Mice were sensitized and challenged with aerosolized OVA twice weekly for up to 82 days. Control mice were treated with PBS. Lung tissue sections were stained through B immunofluorescence for Pref-1 (green), HIF-1α (red), C Fibronectin (green), and PEA3 (red) (original magnification, 20 × ; OVA n = 4, PBS n = 5). Quantification of mean fluorescence intensity (MFI) of D Pref-1, E HIF-1α, F fibronectin, and G PEA3 from B or C. Data are presented as the mean ± S.E.M., *P < 0.05, relative to the PBS group H immunohistochemistry for PEA3 expression (original magnification, 20 × ; OVA n = 5, PBS n = 6). I PEA3 Integrated density are presented as the mean ± S.E.M., *P < 0.05, compared with PBS