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Fig. 5 | Molecular Medicine

Fig. 5

From: The long noncoding RNA MEG3 regulates Ras-MAPK pathway through RASA1 in trophoblast and is associated with unexplained recurrent spontaneous abortion

Fig. 5

MEG3 regulated the expression of RASA1 via EZH2. a Cytoplasmic and nuclear localization of MEG3. b Determination of the ability of EZH2 protein to interact with MEG3 via RIP assay. c Identification of MEG3-bound proteins by RNA pulldown assay. d Verification the direct binding of EZH2 protein and MEG3 using EMSA assay. e and f ChIP assay for the recruitment of EZH2 at the RASA1 promoter gene by MEG3. g and h Analysis of the effects of MEG3 on the levels of H3K27me3 at the RASA1 promoter using ChIP assay. i GA-enriched motifs (chromatin-interacting sequences) at the 5’-end region of MEG3 sequence. Interactions of MEG3 with the promoter sequence of the RASA1 gene using DNA pulldown assay. *P < 0.05

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Molecular Medicine

ISSN: 1528-3658