Skip to main content
Fig. 3 | Molecular Medicine

Fig. 3

From: A CREB1-miR-181a-5p loop regulates the pathophysiologic features of bone marrow stromal cells in fibrous dysplasia of bone

Fig. 3

Low miR-181a-5p expression promotes osteogenic properties in FD BMSCs. a Quantitative real-time PCR analysis of osteogenesis-related marker mRNA expression in BMSCs and FD BMSCs after osteogenic induction for 7 days. b Western blot analysis of osteogenesis-related marker protein expression in BMSCs and FD BMSCs cultured with osteogenesis induction medium for 7 days. c Alizarin red staining and CPC assay showed calcium deposition in BMSCs and FD BMSCs after 14 days of osteogenic induction. Quantitative real-time PCR (d) and western blot (e) analysis of osteogenesis-related gene and protein expression in FD BMSCs transfected with miR-181a-5p mimics. Quantitative real-time PCR (f) and western blot (g) analysis of osteogenesis-related gene and protein expression in BMSCs transfected with the miR-181a-5p inhibitor. The data are presented as the mean ± S.E.M. values (n = 3). #P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page

Molecular Medicine

ISSN: 1528-3658