Fig. 7

SOX2 regulates STAT3 expression via activating PVT1 to sponge miR-24-3p. A Bioinformatics website prediction of the binding site of SOX2 and PVT1 promoter; B Dual luciferase reporter gene assay of validation of the binding relationship between SOX2 and PVT1 in HEK293 cells and RN-c cells; C ChIP-qPCR assays of validation of the binding relationship between SOX2 and PVT1 in HEK293 cells and RN-c cells; D The surmised binding sites for interaction between PVT1 and miR-24-3p; E Dual luciferase reporter gene assay of verification of the binding of PVT1 and miR-24-3p in HEK293 cells and RN-c cells; F RNA-pull down assay of verification of the binding relationship between PVT1 and miR-24-3p in HEK293 cells and RN-c cells; G RIP assay of verification of the binding relationship between PVT1 and miR-24-3p in HEK293 cells and RN-c cells; H The surmised binding sites for correlation between miR-24-3p and STAT3; I Dual luciferase reporter gene assay of validation of the targeting relationship between miR-24-3p and STAT3 in HEK293 cells and RN-c cells; Repetition = 3