Fig. 5
From: rAAV9‐mediated supplementation of miR-29b improve angiotensin-II induced renal fibrosis in mice
Effects of miR-29b gene transfer on renal injury in vivo. A Assessment of apoptosis. Representative epifluorescence images of TUNEL labeling of kidney histological sections at 5 weeks post gene transfer. Scale bar: 50 μm B, Quantification of TUNEL-positive nuclei. Values are mean ± SEM measured from 10 000 nuclei (n = 3). C, D, Renal function was assessed by the level of serum creatinine and urea nitrogen in each group. n = 6. E, tubular injury score in each group. F, G Kidney injury molecule-1 (KIM-1, E) and neutrophil gelatinase-associated lipocalin (NGAL, F) in renal cortex determined by real-time RT-PCR. mRNA values were normalized by the levels of GAPDH mRNA and then expressed relative to the mean value for the control group (n = 6). Values are mean ± SEM. *P < 0.05 vs. Control; #P < 0.05 vs. AngII + rAAV9-Negative control (NC)