Fig. 3

The dose-dependent drug screening of three drugs whose transcriptional profiles correlate negatively with the host transcriptional signature associated with SARS-CoV-2 infection in the connectivity mapping analysis, which were further selected based on their predicted ability to bind to SARS-CoV-2 Mpro and RdRp. A Atorvastatin was the only drug that showed a dose-dependent reduction in viral entry in the SARS-CoV-2 lung organoid model, alongside our positive control (doxycycline). The bar plot shows the relative luciferase activity of multiple candidate drugs at 100 μM, 33 μM and 10 μM. The data was normalized to DMSO treated control. Data is presented as mean ± SEM. Two-way ANOVA, **P < 0.01, ***P < 0.001. B The dose-dependent efficacy curves of atorvastatin, and C doxycycline, suggest that these drugs inhibit viral entry in the SARS-CoV-2 lung organoid model. Their maximum half cytotoxicity concentrations are above the tested range. The data was normalized to DMSO-treated control. Data is presented as mean ± SEM