Fig. 1

A, B Bmal1 and Per2 protein expression measured in renal lysates by ELISA (shown in dashed lines); C, D Bmal1 and Per2 expression measured in whole renal mRNA by qPCR (shown in solid lines)—each symbol represents one mouse;); Ac  time of acrophase, ZT Zeitgeiber time where ZT0 is the time of lights on. E Heatmap of all genes passing the RAIN test for circadian rhythm in young and nephritic kidneys. Complete results from the microarray analysis are on the left and from the RNASeq analysis are on the right. For each time interval, indicated by Zeitgeiber time (ZT0 = lights on, ZT12 = lights off), genes regulated in young mice are shown in the upper panel (blue bar), those regulated in nephritic mice are shown in the middle panel (aqua bar) and those regulated in common between the two groups are shown in the lower panel (purple bar). F–I RNASeq data of circadian transcriptional regulators in young (light and dark blue—F, H) and old (light and dark orange—G, I) C57BL/6 mice at ZT0 (light colors) and ZT12 (dark colors) F, G compared with young (light and dark blue) and nephritic (light and dark orange) NZB/W kidneys H, I—each symbol represents one mouse