Fig. 3

Effect of circ_0007959 overexpression on the apoptosis of IFN-treated RMCs and HEK293 cells. RMC and HEK293 cells were transfected with a circ_0007059-overexpressing vector or NC vector for 24 h, followed by treatment with 1000 units/mL IFN for 24 h. A, B the number of apoptotic cells was evaluated by flow cytometry. In each plot, the lower right quadrant represents the early apoptotic cells. The analysis of the apoptosis rate in each group is shown in the table below (biological replicates, 2; technical replicates, 3; repeat time, 3). C, D WB was performed to detect the expression of apoptotic markers, including the cleavage forms of caspase-3 and -9 (biological replicates, 3; technical replicates, 1; repeat time, 3). Results are expressed as the mean ± SEM for technical replicates. *P < 0.05, **P < 0.01 vs. the indicated group