Fig. 7

Influence of SHP-1 overexpression on viability, apoptosis, and IFN-associated inflammation of RMCs and HEK293 cells. RMC and HEK293 cells were transfected with an SHP-1-overexpressing vector or NC vector for 24 h, followed by treatment with 1,000 units/mL IFN for 24 h. Expression of (A, B) SHP-1 was measured in cells with different transfection constructs by RT-qPCR (biological replicates, 2; technical replicates, 3; repeat time, 3). C, D WB was performed to detect SHP-1, STAT3, and phosphorylated STAT3 levels in RMCs and HEK293 cells (biological replicates, 1; technical replicates, 3; repeat time, 3). E, F cell viability of RMCs and HEK293 cells were determined by MTT assay (biological replicates, 1; technical replicates, 3; repeat time, 3). G, H the number of apoptotic cells was evaluated by flow cytometry (biological replicates, 1; technical replicates, 3; repeat time, 3). I, J expression of mRNA for CXCL10, IFIT1, ISG15, and MX1 gene in RMCs and HEK293 cells as determined by RT-qPCR (biological replicates, 2; technical replicates, 3; repeat time, 3). Results are expressed as the mean ± SEM for technical replicates. *P < 0.05, **P < 0.01 vs. the indicated group