Fig. 7

miR-214 overexpression blocks the effect of the activated STAT3 pathway on bronchial embryonic pulmonary epithelial cells by inhibiting PlGF. A The ultrastructure of alveolar epithelial cells under TEM (× 10,000). B Western blot analysis to quantify the expression of antiapoptotic proteins (Survivin and Bcl-2) and proapoptotic proteins (Bax, c-myc, and cleaved caspase-3) proteins in embryonic pulmonary epithelial cells. C Western blot analysis to quantify the expression of the epithelial cell marker E-cadherin and the fibrosis marker α-SMA in embryonic pulmonary epithelial cells. Data are summarized as mean ± standard deviation. *p < 0.05 vs. pulmonary epithelial cells transfected with miR-214 NC and PlGF NC. ^#p < 0.05 vs. pulmonary epithelial cells transfected with miR-214 NC and PlGF. Multiple comparisons were performed using one-way ANOVA, followed by Tukey's post hoc test. Each experiment was repeated three times