Table 1 Primer sequences specific for TNF-α, IL-6 and IL-1β
Gene | Primer | Length (bp) |
|---|---|---|
TNF-α (sense) | 5’-ACGGCATGGATCTCAAAGAC-3’ | 116 |
TNF-α (anti-sense) | 5’-GTGGGTGAGGAGCACGTAGT-3’ | |
IL-1β (sense) | 5’-GCTGCTTCCAAACCTTTGAC-3’ | 121 |
IL-1β (anti-sense) | 5’-AGCTTCTCCACAGCCACAAT-3’ | |
IL-6 (sense) | 5’-CCGGAGAGGAGACTTCACAG-3’ | 134 |
IL-6 (anti-sense) | 5’- CAGAATTGCCATTGCACAAC-3’ | |
β-actin (sense) | 5’-GTCAGGTCATCACTATCGGCAAT-3’ | 147 |
β-actin (anti-sense) | 5’-AGAGGTCTTTACGGATGTCAACGT-3’ |