Fig. 4

PEGylation increases duration of ChAT-induced blood pressure decrease. A change in normalized MAP. MAP is normalized to the 30-min period before PEG-ChAT or vehicle administration, and area under the curve is calculated from a baseline of 100% in the 3 h post-PEG-ChAT administration. Negative values correspond to decrease in MAP. n = 7–11. ***p < 0.001, 1 mg/kg vs. vehicle. One-way ANOVA with Dunnett’s multiple comparison test. B MAP in 3 h post-PEG-ChAT administration. MAP was compared between PEG-ChAT and vehicle groups at each time point post-administration. n = 7–11. *p < 0.05, two-way ANOVA with Sidak’s multiple comparison test. C–E changes in C heart rate, D temperature and E activity after PEG-ChAT administration. Change is calculated as the baseline (30 min pre-administration) subtracted from the average data in the 3 h post-administration period. Data are represented as individual mouse data points, mean ± SEM. n = 7–11. ns not significant, *p < 0.05, t-test. F MAP in the 24 h post-PEG-ChAT administration. Gray background indicates dark cycle. Data are averaged every hour and represented with lines as group mean ± SEM. n = 7–11. ns: not significant, two-way ANOVA with Sidak’s multiple comparison test. G time course responses to ChAT and PEG-ChAT. Baseline was defined as 30-min average MAP prior to administration. Individual post-administration MAP was normalized to individual pre-administration MAP, and return time was calculated for each animal. Return time was defined as the minimum time after administration where mice returned to baseline MAP for a 10-min average period. Data are represented as individual mouse points, with bars with mean ± SEM. n = 5–11. *p < 0.05, **p < 0.01, one-way ANOVA with Dunnett’s multiple comparison test