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Fig. 3 | Molecular Medicine

Fig. 3

From: lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

Fig. 3

LncRNA SNHG1 knockdown promoted the osteogenic differentiation of BMSCs through regulation of miR-181c-5p. A and B The levels of lncRNA SNHG1 and miR-181c-5p in BMSCs were determined using qPCR at 0, 7, 14 and 21 days after osteogenic induction. BMSCs were transfected with sh-NC, sh-SNHG1, sh-SNHG1 with inhibitor NC, or sh-SNHG1 with miR-181c-5p inhibitor and induced for osteogenic differentiation. C and D ALP staining and ARS were performed at day 14 and 21 after osteogenic differentiation. E–F 21 days after osteogenic induction, the mRNA and protein levels of ALP, OCN and RUNX2 in BMSCs were detected by qPCR and western blot. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658