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Fig. 5 | Molecular Medicine

Fig. 5

From: lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

Fig. 5

Overexpression of lncRNA SNHG1 inhibited tube formation of BM-EPCs via targeting miR-181c-5p. The BM-EPCs were transfected with vector-NC, SNHG1 vector, SNHG1 vector with miR-NC, or SNHG1 vector with miR-181c-5p mimics for 48 h. A and B The cell proliferation and colony were detected by CCK-8 assay and colony formation assay. C After cultured with Matrigel for 6 h, the images of tubular structures of transfected BM-EPCs. D The tube lengths in every group. E The mRNA expression level of VEGF in BM-EPCs was detected by qPCR. D and E The protein expression level of VEGF was measured through western blot. F and G Cell migration was detected using Transwell assay. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658