Fig. 4

The effects of lncRNA-ATB knockdown on the COM-induced EMT in HK‐2 cells. a Morphological Changes of HK-2 cells exposed to 200ug/ml COM or 10 ng/ml TGF-β1 for 48 h under light microscope. b,c The mRNA b or protein c levels of EMT markers in HK-2 cells exposed to 200ug/ml COM or 10 ng/ml TGF-β1 for 48 h. d Quantitative results of EMT marker-protein levels. e The expression levels of E-cadherin and α-SMA were detected by immunofluorescence. All above the experiments were repeated 3 times, values are mean ± SD; *P < 0.05, **P < 0.01 VS. sh-NC group, ^#P < 0.05, ^##P < 0.01 VS. sh-NC + COM group, ^▲P < 0.05, ^▲▲P < 0.01 VS. sh-NC + TGF-β1 group, Scale bars = 200 μm