Fig. 4

rhFGF21 inhibited DOCA-salt-induced renal inflammatory response in mice. Eight-week-old male FGF21 WT mice were randomly divided into three experimental groups: the WT-Control group treated with sham operation, followed by intraperitoneal injection with 0.9% normal saline; the WT-DOCA group treated with DOCA-salt; the WT-DOCA-FGF21 group treated with DOCA-salt, followed by intraperitoneal supplementation of recombinant human FGF21 (rhFGF21, 500 μg/kg/day body weight). Kidney tissues were harvested at 8d after intervention. a Representative images of kidney sections stained with anti-F4-80 antibody, anti-IL-6 antibody and anti-TNF-α antibody, respectively, harvested from mice at 8 days after receiving DOCA-salt treatment. The arrow indicates F4/80 positive cells. b and c Quantifications of IL-6 and TNF-α protein expression in the kidney tissues of each group were determined by using the mean integrated optical density (IOD)/area with Image-Pro plus 6.0 image analysis software. d and e Kidney mRNA expression levels of IL-6 and TNF-α were determined by Q-PCR and values normalized to GAPDH. Data are presented as Mean ± SD, n = 6 mice/group. *P < 0.05, **P < 0.01 versus the WT control group; ^#P < 0.05, ^##P < 0.01 versus the WT-DOCA group. Scale bar: 100 μm