Fig. 6

Effects of colivelin treatment on STAT3 activation and Nestin/Nrf2/HO-1 pathway in Aβ-treated SH-SY5Y cells. Cells were subjected to transfection with a PIAS3 overexpression vector or an empty vector for 1 d, and subsequently subjected to Aβ treatment (20 μM in 0.1% DMSO) and colivelin treatment (0.5 μM in 0.1% DMSO) for an additional 1 d. A–D qPCR was used to determine mRNA level expressions of STAT3, Nestin, Nrf2, and HO-1. E WB was utilized for determining protein level expressions of STAT3, Nestin, Nrf2, and HO-1, and STAT3 phosphorylation in SH-SY5Y cells. F ARE luciferase activity was also determined. Data represent mean ± SEM. **p < 0.01, ***p < 0.001. PIAS3, protein inhibitor of activated STAT3, STAT3 signal transducer and activator of transcription 3, Nrf2 nuclear factor erythroid 2-related factor, HO-1 heme oxygenase-1, qPCR quantitative polymerase chain reaction, Aβ amyloid beta, WB western blotting, ARE antioxidant responsive element, DMSO dimethyl sulfoxide