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Fig. 7 | Molecular Medicine

Fig. 7

From: Metformin alleviates the calcification of aortic valve interstitial cells through activating the PI3K/AKT pathway in an AMPK dependent way

Fig. 7

AKT inhibition ameliorates the anti-calcification and anti-apoptosis effect of metformin on AVICs. a Protein expression levels of the PI3K/AKT and apoptosis pathway in AVICs after various treatments with metformin (100 μM) and AKT inhibitor (MK2206, 5 μM) for 72 h as determined by WB. c Protein expression levels of the PI3K/AKT and apoptosis pathway in AVICs after various treatments with metformin (100 μM) and AKT siRNA for 72 h as determined by WB; b and d Calcium deposition were detected by ARS Staining after various treatments for 7 days, Scale bar, 200 μm. n = 6 per group. CTL control; PM phosphate medium; Met metformin; *p < 0.05 versus PM group; #p < 0.05 versus Met group (one-way ANOVA with Bonferroni post hoc test)

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Molecular Medicine

ISSN: 1528-3658