Fig. 3

miR-1-3p down-regulates Notch3 and promotes LPS-induced intestinal epithelial cell injury. A The heatmap of differentially expressed genes in sepsis obtained from the profile GSE53007. B Volcano plot of differentially expressed genes in sepsis. C The intersection of target genes of miR-1-3p predicted by starbase, mirDIP, and miRDB databases and down-regulated genes in sepsis from GSE53007. D The miR-1-3p binding sites in Notch3. E The expression of Notch3 in normal and sepsis samples from GSE53007. F The relationship between miR-1-3p and Notch3 as examined by dual-luciferase reporter gene assay. G The effect of miR-1-3p on Notch3 mRNA expression as determined by RT-qPCR. H The effect of miR-1-3p on Notch3 protein expression as determined by Western blot assay. I The expression of Notch3 in LPS-induced cells as determined by RT-qPCR. J The silencing effect of miR-1-3p and sh-Notch3 as examined by RT-qPCR and Western blot assay. K The expression of miR-1-3p and Notch3 in LPS-induced cells treated with miR-1-3p inhibitor or miR-1-3p inhibitor + sh-Notch3 as determined by RT-qPCR. L The levels of TNF-α, IL-1β and IL-6 in supernatant of LPS-induced cells treated with miR-1-3p inhibitor or miR-1-3p inhibitor + sh-Notch3 as determined by ELISA. M Determination of ROS and MDA levels and SOD activity in LPS-induced cells treated with miR-1-3p inhibitor or miR-1-3p inhibitor + sh-Notch3. N The apoptosis of LPS-induced cells treated with miR-1-3p inhibitor or miR-1-3p inhibitor + sh-Notch3 as examined by TUNEL staining. O The expression of cleaved-caspase-3 and caspase-3 in LPS-induced cells treated with miR-1-3p inhibitor or miR-1-3p inhibitor + sh-Notch3 as determined by Western blot assay. *p < 0.05. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was performed for the data analysis between two groups while one-way ANOVA with Tukey's test for data analysis among multiple groups. Experiment was repeated independently three times