Fig. 4

LDs accumulation in neuron. To detect LDs accumulation in the hippocampal neurons, Nile red (red) and DAPI (blue), BODIPY (483/503) (green) and NeuN (red) were used. A LDs accumulation could be detected (red, black arrow) in the WT + CIH mice, and treated with SMND-309 decreased this change. B Abundant BODIPY (483/503) fluorescence could be detected in the neurons of the DG area under CIH condition, but the fluorescence became dim after SMND-309 treatment. C Quantification of LDs accumulation in nerve and glia cells. D Quantification of LDs accumulation in neurons. *p < 0.05 WT group vs. WT CIH group. **p < 0.05 WT CIH group vs. SMND-309 CIH group. All experiments were repeated three times. Data are shown as the mean ± SEM. Statistical analysis was tested by two-way ANOVA. Original magnification: 400×