Fig. 2

Decreased ISL1 was observed in NAFLD. A Oil red O staining was applied to detect liver lipid accumulation in mice exposed to high-fat diet. B Morphology of liver tissues detected by HE staining. C Detection of plasma contents of TG and TC in mice exposed to high-fat diet. D Detection of contents of TG and TC in liver tissues of mice exposed to high-fat diet. E Western blot analysis was conducted to detect the expression of ISL1, lipid synthesis- and lipolysis-related genes in liver tissue of mice exposed to high-fat diet. F Immunohistochemistry was used to detect the expression of ISL1 in liver tissues of mice exposed to high-fat diet. G Oil red O staining was performed to detect lipid accumulation. H Western blot analysis was used to detect the expressions of ISL1, lipid synthesis- and lipolysis-related genes in an in vitro model of lipid accumulation. **p < 0.01 vs. Control (cells without treatment); ***p < 0.001 vs. Normal (mice on standard diet) or Control (cells without treatment). n = 8 mice in each group