Fig. 3

ISL1 led to inhibited lipogenesis and induced lipolysis. A Western blot analysis was used to detect the ISL1 overexpression efficiency in liver tissues of mice exposed to high-fat diet. B Oil red O staining was applied to detect the effect of overexpressed ISL1 on liver lipid accumulation in mouse model. C Morphology of liver tissues detected by HE staining. D Effect of overexpressing ISL1 on plasma contents of TG and TC in mice exposed to high-fat diet. E Effect of overexpressing ISL1 on the contents of TG and TC in liver tissues of mice exposed to high-fat diet. F Western blot analysis was conducted to detect the effect of ISL1 on lipid synthesis- and lipolysis-related genes in liver tissues of mice exposed to high-fat diet. G Oil red O staining was performed to detect the effect of ISL1 on lipid accumulation. H Western blot analysis was used to detect the effect of ISL1 on the expressions of lipid synthesis- and lipolysis-related genes in an in vitro model of lipid accumulation. **p < 0.01, ***p < 0.001 vs. oe-NC treatment. n = 8 mice in each group