Fig. 8

Cold-induced mitochondrial fission despite the presence of the Drp1 inhibitor Mdivi-1. Porcine aortic endothelial cells (control, A, I) were incubated at 4 °C for 3 h, 6 h and 24 h with 20 µM Mdivi-1 (E–H, M–P), a potent Drp1 inhibitor. DMSO was added as solvent control (A–D, I–L). Mitochondria were stained with MitoTracker Red. Additionally, Drp1 immunofluorescence (green) staining was applied (I–P). The images were documented by fluorescence microscopy (MitoTracker Red: λ_exc. = 546 ± 6 nm, λ_em. ≥ 590 nm; FITC: λ_exc. = 470 ± 20 nm, λ_em. = 525 ± 25 nm). Representative figures of n = 4 experiments. For quantification of mitochondrial fission (Q) cells were categorised in cells with predominantly short, intermediate and long mitochondria by a person blinded to the experiment. Means ± SD of n = 4 experiments