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Fig. 3 | Molecular Medicine

Fig. 3

From: Long noncoding RNA XIST regulates brown preadipocytes differentiation and combats high-fat diet induced obesity by targeting C/EBPα

Fig. 3

Silencing of XIST inhibits brown preadipocyte differentiation. A–B, D–E, G–H qRT-PCR analysis of XIST and indicated genes during the differentiation of brown preadipocytes transfected with control or XIST smart silencer. C, F, I Western blot analysis of the expression of indicated proteins during the differentiation of brown preadipocytes transfected with control or XIST smart silencer. J–K qRT-PCR analysis of XIST and indicated genes in differentiated brown preadipocytes transfected with control or XIST smart silencer. L RNA-binding protein immunoprecipitation (RIP) assay showing the interaction between XIST and C/EBPα. *, p < 0.05. Data are represented as mean ± SEM

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Molecular Medicine

ISSN: 1528-3658