Fig. 6

β-catenin disturbance and mesenchymal transition of HUVEC with increased barometric pressure. HUVECs exposed to increased barometric pressure were treated with control solvent DMSO or pyrvinium pamoate. The negative control group received DMSO treatment only. A–C IF images double stained for β-catenin and αSMA. D Quantification of αSMA expression. E Quantification of β-catenin nuclearization. Data are presented as median, 1st and 3rd quartiles, maximal and minimal values in the box plots (n = 5 per group). P values in D, E were determined by the Wilcoxon signed-rank test with Bonferroni correction. Insignificant P values are not shown. Images were obtained using the TissueFAXS system. HUVEC human umbilical endothelial venous cell, DMSO dimethyl sulfoxide, αSMA smooth muscle alpha-actin, Arbitrary unit (AU) = integrated density/area