Fig. 6
Eriodictyol inhibits ferroptosis induced by the Aβ1–42 oligomer in HT-22 cells. A The levels of ferrous iron and total iron in HT-22 cells were detected using an iron assay kit. B The iron content in HT-22 cells was determined using an immunofluorometric assay with calcein-AM.C The expression of TfRC, FTH, and Fpn was measured using Western blotting. D The relative mRNA expression levels of these proteins were tested using q-PCR. E ROS levels in HT-22 cells were measured using two types of fluorescent probes (DHE and DCFH-DA) and F the MDA content in HT-22 cells was measured using a Lipid Peroxidation MDA Assay Kit. G GPX4 protein levels in HT-22 cells were detected using Western blotting and H its relative mRNA expression level was measured using q-PCR. I The morphological features of mitochondria in HT-22 cells were observed under an electron microscope. The data are presented as the means ± SD of three experiments. **P < 0.01, and ***P < 0.001 compared with the Aβ1–42 oligomer group. ##P < 0.01, ###P < 0.001 compared with the control group