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Fig. 9 | Molecular Medicine

Fig. 9

From: Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson’s disease models in vitro and vivo

Fig. 9

The mitochondrial oxidative phosphorylation function in right substantia nigra of rotenone-induced PD mouse model transduced with NDI1. A The mitochondrial complex I enzyme activity was determined by measuring the NADH oxidation rate using a spectrophotometer. B The total oxygen consumption of mitochondrial complexes I and II (C I + C II), ATP synthase uncoupling oxygen consumption after oligomycin treatment (Oligo), and maximum oxygen consumption after FCCP treatment (FCCP) were detected using a cellular respiration apparatus. C RCRs (respiratory control rates) were calculated as (C I + C II)/Oligo. D LCRs (leakage control rates) were calculated as Oligo/FCCP. Rotenone + vector group compared with DMSO + vector group, or Rotenone + NDI1 group compared with Rotenone + vector group, or Rotenone + NDI1 group compared with DMSO + vector group, ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658