Fig. 8

2′,3′,4′-THC inhibits E2 activation of the c-MYC gene. A MCF-7 cells were treated with 10 nM E2, 5 μM 2′,3′,4′-THC, and 5 μM Tam alone or in combination for 2 h and the levels of c-MYC mRNA were measured by qRT-PCR. The error bars are means ± SE. B 2′,3′,4′-THC inhibits E2 stimulation of c-MYC protein levels. The cells were treated with 10 nM E2 alone or in combination with increasing doses of 2′,3′,4′-THC (THC) or Tam for 3 h and c-MYC was determined by western blotting. C Quantification of c-MYC protein levels in cells treated with E2 and 2′,3′,4′-THC or Tam. D 2′,3′,4′-THC inhibits E2-induced ERα recruitment to c-MYC enhancer region. The cells were treated with 10 nM E2 without or with 5 μM 2′,3′,4′-THC for 1 h and ERα binding was determined by ChIP assay. Each bar represents the mean of triplicate samples ± SE. Asterisks over bars show the significance between E2 alone and various treatments determined by one-way ANOVA followed by Tukey's multiple comparisons post hoc test