Fig. 4
From: EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes
EGF induces the expression of oligodendrogenic genes through Erk pathway during Sox10-based reprogramming. A Gene expression of Olig1 and Olig2 after 36 h of EGF and EGF + U0126 treatment. B Western blotting analysis of p-Erk1/2 and Erk1/2 after 2 h of EGF, EGF + Gefitinib and EGF + U0126 treatment. C Quantitative analysis of p-Erk1/2 and Erk1/2 protein expression in cultures presented in B. Histograms express results in arbitrary units, using Ctrl cell values as 100%. D Quantification of the percentage of O4+ cells in reprogramming cultures treated with EGF or EGF + U0126. Statistical analyses are presented as Mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001