Fig. 4
From: Antidotal treatment of botulism in rats by continuous infusion with 3,4-diaminopyridine
Time-dependent recovery of diaphragm endplate potentials (EPPs) in 3,4-DAP infused intoxicated rats. Diaphragm endplate success rates, quantal content (QC) and miniature EPPs (mEPPs) were compared among naïve rats (black); rats intoxicated with 0.44 ng/kg BoNT/A, infused with 3,4-DAP from 1 to 14 d at 1.0 mg/kg∙h (green) or 1.5 mg/kg∙h (pink) and euthanized at 21 d; or rats intoxicated with 0.44 ng/kg BoNT/A, infused with 1.5 mg/kg∙h 3,4-DAP from 1 to 5 d and euthanized (blue). A Histogram of EPP success rates. During each endplate recording, phrenic nerves were stimulated with a train of 10 impulses at 0.2 Hz and the percentage of impulses that produced EPPs were determined. B–E Mean ± SEM scatter plots for (B) Quantal content, (C) mEPP frequencies and (D) mEPP amplitudes. For B and D, each circle represents average responses of 10 stimulations in one endplate. For C, each circle represents mEPP frequencies measured in one endplate. ****p < 0.0001, ns indicates significant