Fig. 2

Effects of hypoxia on the apoptosis of OA-FLSs or RA-FLSs. A After exposed to different oxygen concentrations for 24 h, cells were analyzed by flowcytometry using AV/PI double-staining; B Histogram depicts the quantitative data from the AV/PI double-staining (mean ± SD, n = 6 from three independent experiments); C Representative fluorescence images of cells stained with TUNEL and PI to visualize apoptosis cells (Green) and nucleus (Blue), respectively; D The expression of Bax and Bcl-XL was analyzed by Western blot (mean ± SD, n = 6 from three independent experiments). ^#P < 0.05, ^##P < 0.01 represents the comparison between OA-FLSs N and RA-FLSs N; *P < 0.05, **P < 0.01 represents the comparison between OA-FLSs N and OA-FLSs H, ⁺P < 0.05, ⁺⁺P < 0.01 represents the comparison between RA-FLSs N and RA-FLSs H, ^ΔP < 0.05, ^ΔΔP < 0.01 represents the comparison between OA-FLSs H and RA-FLSs H.