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Fig. 2 | Molecular Medicine

Fig. 2

From: Evaluation of serum tRF-23-Q99P9P9NDD as a potential biomarker for the clinical diagnosis of gastric cancer

Fig. 2

Methodological evaluation of tRF-23-Q99P9P9NDD. A, B Room temperature placements and repeated freeze–thaw experiments showed no significant changes in the expression level of tRF-23-Q99P9P9NDD. C Evaluation of RNA sample integrity by agarose gel electrophoresis. D Evaluation of RNA sample integrity by RIN evaluation. E The product of qRT-PCR was electrophoresed on 2% agarose gel, and a single band of about 80 bp was obtained. F Sanger sequencing of the qRT-PCR product confirmed that the product contained the full-length sequence of tRF-23-Q99P9P9NDD

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