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Fig. 3. | Molecular Medicine

Fig. 3.

From: Preparation of a miR-155-activating nucleic acid nanoflower to study the molecular mechanism of miR-155 in inflammation

Fig. 3.

A Gel image of the product after the complexation of NFs and PEI. Lane 1: PEI-NFs complexes; lane 2: NFs; lane 3: PEI; lane 4: negative control (The loading amount was the same for both (5 µL), and the amount of nucleic acid was 0.15 µg/µL). B Cellular viability of HUVECs exposed to saRNA and PEI-NFs for 24 h. “0” represents the control. C Fluorescence imaging of cells treated with PEI-NF complex for 24 h (magnification, 10 ×). D Flow plots at 12 h after the NFs entered the cells. Results for the control group and experimental group are shown. E Efficiency of PEI-NF delivery into cells after 12 h (****P < 0.0001). F Relative VEGF expression in cells treated with saRNA or PEI-NFs complex for 72 h (**P < 0.01 vs control).

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Molecular Medicine

ISSN: 1528-3658