Fig. 1

mRNA expression of kinases does not correlate to kinase activity. A Schematic representation of the workflow. Kinome profiles were established, and RNA sequencing data were acquired for patient-derived CCA organoids (CCAOs). Upstream kinases were identified from the Uniprot and Ensemble databases for the peptides included in the kinome profiling array. Correlation analysis was performed to investigate the relationship between mRNA expression levels and kinase activity. Schematic created with BioRender.com. B Violin plots with box plots of the RNA expression levels show a similar overall distribution of genes in the three CCAO lines. C Heatmap of the activity (AUC of the activity plots) and mRNA expression (normalized counts) of the 37 upstream kinases included in the kinome profiling array in the three CCAO lines. Data is shown by calculated z-score. D Scatter plots demonstrate there is no correlation between the kinase activity and RNA expression level of the upstream kinases in the three CCAOs.