Fig. 1

LncRNA expression profile in crushed sciatic nerves. A Heatmap of DElncRNAs between crushed sciatic nerves (n = 3) and intact contralateral nerves (n = 3) 4 dpi. B qRT-PCR analysis of lncARAT levels in crushed sciatic nerves (n = 5) at different time points postinjury (0, 1, 4, 7, 14, 21, and 28 dpi). C Immunofluorescence (IF) analysis of lncARAT expression in cross sections of sciatic nerves 0, 7, and 21 dpi. Scale bar, 50 µm. D In situ hybridization analysis of lncARAT (red) on cross sections of sciatic nerve tissues at 4 dpi. Scale bar, 20 µm. S100 β (green) was used to indicate SCs. IF (E) and western blot (F) analysis of SCG10 in injured nerves after lncARAT overexpression or knockdown at 3 dpi. Scale bar, 50 µm. G Quantification of latencies and amplitudes of the compound muscle action potential (CMAP) after lncARAT overexpression or knockdown (n = 5). H Plot of SFI obtained with walking track analysis after sciatic nerve crush in lncARAT overexpression or knockdown rats (n = 5 per group). *p < 0.05. **p < 0.01