Fig. 6

MiRNA-329-5p regulates macrophage activation by targeting SOCS2-STAT1/6 signaling. qRT-PCR (A) and western blot (B and C) analysis of IL-10, CD206, and Arg1 expression in U937 macrophages after miRNA-329-5p inhibition for 48 h. D A Venn diagram analysis of overlapping mRNAs in both sets of data. E KEGG analysis of DEmRNAs between crushed sciatic nerves and intact contralateral nerves. F Schematic representation of the miRNA-329-5p site in the SOCS2-3ʹUTR. G Luciferase activity was assayed in HEK293 cells after cotransfection with miRNA-329-5p and luciferase reporters containing the SOCS2-3ʹUTR. H and I Western blot and quantitative analysis of SOCS2 in U937 macrophages after miRNA-329-5p inhibition. J qRT-PCR analysis of IL-10, CD206, and Arg1 expression in U937 macrophages after miRNA-329-5p inhibition in the presence or absence of siSOCS2. K–N Western blot analysis of p-STAT1, p-STAT3, and p-STAT6 in U937 macrophages after SOCS2 overexpression for 48 h. *p < 0.05. **p < 0.01