Fig. 3

Hyperphosphorylation of soluble and insoluble tau one month after anesthesia in COMTKO/P301L mice. Total (DA31) and phosphorylated tau (ptau) were quantified with ELISA, and the ptau/total tau ratios were compared. A, B In the soluble fraction of mice that were anesthetized when 4-month-old, phosphorylation at the PHF1 epitope was increased in the cortex (A), while that of RZ3 was decreased in the hippocampus (B). C In the insoluble fraction of the cortex, no differences were found. n per group = 12. Data are mean ± SEM. Student’s t-test was performed. *p < 0.05, **p < 0.01. C, Controls; 1 M, one-month post-anesthesia; mo, month-old