Fig. 1

Expression of lncRNA H19 and HDACs in db/db mice skeletal muscle. A Total RNA was isolated from the skeletal muscle of normal (db/ +) and diabetic (db/db) mice and 1 µg RNA was reverse transcribed and subjected to qRT-PCR to assess the transcript levels of the lncRNA H19. 18S rRNA was used as the loading control. Skeletal muscle of normal (db/ +) and diabetic (db/db) mice were lysed as described in the “Material and methods” section and 20–30 µg lysates were run on SDS-PAGE and the levels of HDAC 1–6 were evaluated by Western Blot analysis. HSC70 was used as the loading control. Densitometric analyses of the expression are shown in the panel below (B–G). All experiments were performed in at least four animals in each group, values are means ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001