Fig. 1

PAH increased the expression of FDPS, PCNA and Beclin1 in vivo and in vitro. A RVSP and RV/(LV + IVS) in control and MCT groups. n = 4–6 per group. B Hematoxylin and eosin staining of lung tissues. The index of wall thickness was calculated and showed in right. Scale bar = 50 μm. n = 11–12 per group. C Immunoblot analysis for FDPS, PCNA and Beclin1 in control and MCT-induced PAH rats. GAPDH was used as internal reference. n = 4–7 per group. D The mRNA level of FDPS and Beclin1 in PAECs after different concentration of MCTP treatment for 24 h was detected. n = 3 per group. E The mRNA level of FDPS and Beclin1 in PAECs after 50 μm MCTP treatment for different times was detected. Quantitative RT PCR (qRT-PCR) was conducted to measure mRNA level changes, with 18S as an internal control. n = 3 per group. F Immunoblot analysis for FDPS, PCNA and Beclin1 in the control and MCTP treatment PAECs. GAPDH was used as internal reference. n = 3 per group. Data are expressed as the mean ± SEM. All experiments were independently replicated in triplicate. *P < 0.05