Fig. 3

Inhibition of FDPS decreased the proliferation and migration of MCTP-induced PAECs, increased the NO level in MCTP-induced PAECs. A CCK8 assay on cell proliferation at PAECs. n = 5 per group. B NO assay was performed to detect NO concentrations in culture medium and cell lysates. Relative NO concentration in cell lysates was normalized based on total cellular protein concentrations. n = 4 per group. C Crystal violet staining of PAECs that crossed the polycarbonate membrane of the Transwell invasion chamber. Representative photomicrographs were shown in left. The number of migrated cells were calculated and showed in right. n = 19–20 per group. D The scratch assay following 50 μM MCTP and 100 μM IB treating for 24 h in PAECs. Representative photomicrographs were shown in left. The index of migration was calculated and showed in right. n = 7–8 per group. E The relative gene expression of FDPS decreased in the siFDPS‐transfected PAECs. qRT-PCR was conducted to examine mRNA level changes with 18S as an internal control. n = 6 per group. F Immunoblot analysis for FDPS. GAPDH was used as internal reference. n = 6 per group. G Knockdown of FDPS elevated NO production of MCTP-induced cell. n = 6 per group. H The scratch assay following 50uM MCTP treating for 24 h in PAECs and si FDPS-transfected PAECs. Upper showed in vitro scratch assay. The index of migration was calculated and showed in below. n = 8–9 per group. Data are expressed as the mean ± SEM. All experiments were independently replicated in triplicate. *p < 0.05