Fig. 4

Inhibition of FDPS decreased the autophagy level in vivo and in vitro PAH module. A Immunoblot analysis for LC3b, Beclin1 and P62 in the control, MCT-induced PAH rats and IB treated PAH rats. GAPDH was used as internal reference. n = 4 per group. B Inhibition of FDPS decreased the MCTP induced autophagy in PAECs. PAECs transfected with Ad-mRFP-GFP-LC3 was observed and analyzed by microscopy. The representative images are displayed at left. Scale bars = 20 μm. The numbers of green (GFP signal) and red (mRFP signal) puncta per cell were counted. n = 7–8 per group. C Immunoblot analysis for P62 in the control, MCTP treated PAECs and IB treated PAECs. GAPDH was used as internal reference. n = 3 per group. D Knockdown FDPS decreased the MCTP induced autophagy in PAECs. The representative images are displayed at left. Scale bars = 20 μm. The numbers of green (GFP signal) and red (mRFP signal) puncta per cell were counted. n = 7–9 per group. E Immunoblot analysis for P62 in the scramble, MCTP treated scramble PAECs and IB treated siFDPS transfected PAECs. GAPDH was used as internal reference. n = 3 per group. Data are expressed as the mean ± SEM. All experiments were independently replicated in triplicate. *p < 0.05