Fig. 4

eCIRP increases the adhesion of neutrophils to endothelial cells. A Neutrophils on the transwell membrane recovered from the TEM assay were counted by using wide field microscopic images and fiji ImageJ. Scale bar, 100 µm. The cell number of neutrophils per field in PBS or eCIRP treated transwell membrane were quantified. Data represented as mean ± SEM. *p < 0.05, unpaired, two-tailed student’s t-test. B Representative images of neutrophil adhesion to the endothelial cells showing CD11b on the neutrophils (red color) co-localized with plasma membrane staining signal of the endothelial cells (green color) on the transwell membrane after TEM assay. The arrows in the CD11b and the merge panels indicate polarization of CD11b with the plasma membrane staining on the neutrophils. Scale bar, 20 µm