Fig. 6

Trogocytosis of neutrophils is observed in injured Lung. The acute lung injury model was used to detect the trogocytosis of neutrophils. The mice were challenged with LPS by direct intra-tracheal instillation. The neutrophils from mouse lungs were collected by bronchoalveolar lavage (BAL). A To show the trogocytosis of neutrophils in mouse lung tissue, the purified neutrophils from BAL fluid were subjected to antibody staining with anti-Ly6G, anti-JAM-C, and anti-VE-cadherin antibody. The nucleus of the cells was stained with Hoechst33342. Scale bar, 10 µm. B The neutrophils were also subjected to immunofluorescence staining with anti-JAM-C antibody and anti-CD11b antibody to show co-localization of the two markers in the neutrophils. Scale bar, 10 µm. C The trogocytosis of purified neutrophils were quantified by observing the cells (over 500 cells each for BMN and BAL neutrophils) with confocal microscopy. The number of trogocytosis was determined by observing JAM-C positive (+ve) puncta on the edge of cells. Data are shown in percentages