Fig. 7

Gene expression of mitochondrial biogenesis and TFEB pathways in MERRF fibroblasts treated with rapamycin. Wt-fibroblasts, I-fibroblasts and H-fibroblasts were treated four weeks with 20 nM rapamycin. Gene expression of PGC-1α (A), NRF1 (B), ND1 (C), COX2 (D), ATP6 (E), CTSD (F), LAMP1 (G), BECLIN (H) and p53 (I) evaluated by qPCR. ACTIN was used as reference gene. Data are normalized to untreated cells and are means and SD of four biological replicates for Wt-F and three biological replicates for H-F. Only one experiment is reported for I-F. Statistical analyses were performed using unpaired two-tail T-test. P value: *p < 0.05, **p < 0.01, ***p < 0.001